Wastewater Benzenediol Removal Catalyzed by Crude Arugula Peroxidase

Abstract

This study involved the extraction and purification of peroxidase enzymes from arugula. The enzymatic removal of benzenediol (BZOL) compounds from wastewater was investigated using crude arugula peroxidase (AP). Based on the results, pH 6.1 and pH 5.7 were identified as the optimal pH values for the removal of resorcinol and catechol, respectively. The optimum enzyme activity and hydrogen peroxide were found to be at the lowest feasible limits (95% elimination) of 0.15% hydrogen peroxide and 0.072U/mL enzyme activity under reaction conditions (3 hours). Kinetic studies revealed that the reaction followed pseudo-first-order kinetics, with catechol degradation occurring faster than resorcinol degradation. These findings are promising for environmentally sustainable and robust bioremediation applications.