Assessment of Bioefficacy of Achromobacter xylosoxidans KUESCCHK-6 Isolated from Textile Contaminated Soil in Treating Textile Effluent and its Impact on Vigna mungo

Abstract

Textile effluents are major pollutants with varied contaminants. Traditional treatment methods are costly and produce sludge, necessitating alternative, eco-friendly solutions. Biological treatment methods are receiving attention as it is proven to be cheap, environment friendly and highly efficient treatment method for dye effluent in industrial scale as compared to the other available treatment methods. The present work evaluates the bioremediation of textile effluent using a pure culture of a bacterium isolated from the soil samples contaminated with textile wastewater. The strain was identified as Achromobacter xylosoxidans KUESCCHK-6 (GenBank Accession Number: OM475749) through 16S rRNA molecular analysis. This bacterial strain was used to treat textile effluent under specific conditions: glucose as the carbon source, urea as the nitrogen source, a C/N ratio of 6:1, a temperature of 35°C, a pH of 8.5, and a static incubation period of 5 days. The results indicated that the strain effectively reduced various physiochemical parameters of the raw textile wastewater: color by 87.94%, BOD by 80.61%, COD by 80.96%, EC by 73.11%, fluoride by 81.15%, phosphate by 79.57%, sodium by 76.88%, and turbidity by 81.02%. Additionally, metal ions including iron were removed by 84.83%, while other metals such as zinc, nickel, manganese, copper, lead, cadmium, total chromium, arsenic, barium, cobalt, and boron were reduced to below detectable limits. Phytotoxicity tests confirmed the non-toxic nature of the treated effluent. Overall, the study concludes that Achromobacter xylosoxidans KUESCCHK-6 is a promising candidate for the bioremediation of textile industrial effluents, with potential for commercial application.