Eco-Friendly Degradation Of Textile Azo Dye Reactive Orange ME2RL Using Green-Synthesized Iron Nanoparticles From Pseudomonas Stutzeri

Abstract

In this study, iron nanoparticles derived from Pseudomonas stutzeri were found to effectively degrade Reactive Orange me2RL, a synthetic azo dye widely utilized in the textile industry, addressing significant health and environmental issues concerning its genotoxic, cancerous, and mutagenic properties. Interestingly, a degradation efficiency of 99.22% was obtained in only 45 minutes. The iron nanoparticles were synthesized at ambient temperature using ferric chloride as a precursor. The nanoparticles were evaluated using several methods, including UV-visible spectroscopy, FTIR, SEM, EDX, HRTEM, and DLS. Phytotoxicity and fish toxicity studies, along withUV–visible spectroscopy, FTIR, HPLC analysis, and GC–MS,were conducted to confirm the degradation of Reactive Orange Me2RL. Metabolite degradation was the end outcome of the degradation process.The ability of these green-synthesized iron nanoparticles to successfully decompose large concentrations of the dye in a short period of 45 minutes suggests their potential for use in the bioremediation and breakdown of textile dyes.